Highly Selective Uricase-Based Quantification of Uric Acid Using Hydrogen Peroxide Sensitive Poly-(vinylpyrrolidone) Templated Copper Nanoclusters as a Fluorescence Probe

We reported on uric acid (UA) detection using a new fluorescence-based assay: poly-(vinylpyrrolidone) templated copper nanoclusters (PVP-CuNCs) with uricase in an aqueous medium, such as human urine uricase. These were synthesized simple wet chemical method and their morphological optical properties examined the aid of high-resolution transmission electron microscopy absorbance/emission spectroscopy. The PVP-CuNCs acted fluorescence indicators that used enzyme-catalyzed oxidation UA Adding into hybrid PVP-CuNCs/uricase solution caused to occur, producing hydrogen peroxide (H2O2), allantoin, carbon dioxide. intensity is decreased by this biocatalytically generated H2O2, decrease proportional level. A calibration plot showed linear relationship negative slope between range 5–100 × 10−7 mol/L. limit (LOD) was estimated 113 10−9 This fluorescent probe turned out be highly specific for over other biologically relevant molecules. demonstrated capability nanoprobes quantification levels samples could potentially pave way toward medical applications where super-sensitive, cost-effective, UA-specific diagnosis required.